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Product Info Summary
| SKU: | M00144-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290
SKU/Catalog Number
M00144-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290 (Catalog # M00144-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00144-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM290
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD11b (Integrin alpha-M)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD11b (Integrin alpha-M)
Reactive Species
M00144-2 is reactive to ITGAM in Human
Applications
M00144-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
127.179kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:200-1:500 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HL60 cell lysates, nontreated (-) or treated (+) with DMSO using anti-CD11b rabbit monoclonal antibody (clone RM290) at a 1:400 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using anti-CD11b rabbit monoclonal antibody (clone RM290) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127.179kDa
Superfamily
integrin alpha chain family
Alternative Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC 1; MAC1; MAC-1; Mac1, alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha polypeptide; Mo1, alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6 ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 antigen-like family member B|CR-3 alpha chain|antigen CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290 (M00144-2)
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No publications found for M00144-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290
Question
Does anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to the expression profile of liver, ITGAM is highly expressed in liver. So, it is likely that anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 will work for IHC with liver.
Boster Scientific Support
Answered: 2020-04-28
Question
Is this M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 reactive to the isotypes of ITGAM?
P. Carter
Verified customer
Asked: 2020-04-02
Answer
The immunogen of M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 is A peptide corresponding to the C-terminus of human CD11b (Integrin alpha-M). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-02
Question
Is a blocking peptide available for product anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 (M00144-2)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 (M00144-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 (M00144-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
Does M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Mangal
Verified customer
Asked: 2019-08-26
Answer
As indicated on the product datasheet, M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-26
Question
We have been able to see staining in human blood. What should we do? Is anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
From what I have seen in literature blood does express ITGAM. From what I have seen in Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-06-12
Question
I am interested in using your anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 for neutrophil degranulation studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
I appreciate your inquiry. This M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 is tested on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-04
Question
Please see the WB image, lot number and protocol we used for liver using anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
Can you help my question with product M00144-2, anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-26
Question
Our lab were well pleased with the WB result of your anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
From literature, liver does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2018-07-30
Question
I am looking for to test anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 on human liver for research purposes, then I may be interested in using anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The products we sell, including anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-06
Question
I see that the anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-29
Question
Our lab used your anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
I have checked the website and datasheets of our anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-02-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2. Let me know if you need anything else.
M. Wu
Verified customer
Asked: 2013-10-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-16
Question
Is there a BSA free version of anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 available?
W. Huang
Verified customer
Asked: 2013-09-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 M00144-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-11
Question
I was wanting to use your anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
G. Li
Verified customer
Asked: 2013-07-31
Answer
It shows on the product datasheet, M00144-2 anti-CD11b (integrin alpha-M) Rabbit Monoclonal antibody, Clone#RM290 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-31
